14 CFR 121.555 : Aeronautics And Space (§ 1.1 To Parts 1311-1399) — (§ 110.1 To Part 199) — Federal Aviation Administration, Department Of Transportation (Continued) (§ 110.1 To Part 199) — Air Carriers And Operators For Compensation Or Hire: Certification And Operations (§ 110.1 To § 139.343) — Operating Requirements: Domestic, Flag, And Supplemental Operations (§ 121.1 To Appendix P To Part 121) — Flight Operations (§ 121.531 To § 121.590) — Compliance with approved routes and limitations: Domestic and flag operations.

4 Aug 2016

No pilot may operate an airplane in scheduled air transportation -

(a) Over any route or route segment unless it is specified in the certificate holder's operations specifications; or

(b) Other than in accordance with the limitations in the operations specifications.

14 CFR 121.555
[Doc. No. 6258, 29 FR 19219, Dec. 31, 1964, as amended by Amdt. 121-253, 61 FR 2614, Jan. 26, 1996]